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Boehringer Mannheim dig rna labeling kit
Dig Rna Labeling Kit, supplied by Boehringer Mannheim, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dig+labeling+kit/sp6+t7+transcription+kit/pmc09884262__11033_2022_7693_MOESM2_ESM-70-70-76
Average 90 stars, based on 1 article reviews
dig rna labeling kit - by Bioz Stars, 2026-09
90/100 stars

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Related Articles

Labeling:

Article Title: Gene expression for extracellular matrix proteins in shockwave-induced osteogenesis in rats.
Article Snippet: To clarify the mechanisms underlying shockwave-induced osteogenesis, we applied shockwave to rat femoral shafts from the ventral side.. We assessed bone mineral content (BMC) and bone mineral density (BMD), and analyzed the spatial and temporal gene expression for pro-a1 (I) collagen (COL1A1), pro-a1 (II) collagen (COL2A1), pro-a1 (X) collagen (COL10A1), osteocalcin (OC) and osteopontin (OPN) using in situ hybridization.. On the 21st day post-exposure, BMC and BMD in the exposed femur were elevated by 8.46% and 5.80%, respectively, relative to the unexposed femur.

Article Title: Retinoic acid influences the development of the inferior olivary nucleus in the rodent.
Article Snippet: .. Mouse OL-protocadherin (Hirano et al., 1999), and human Brn 3a probes (a gift from Dr. Mengqing Xiang, UMDNJ-Robert Wood Johnson Medical School, NJ) were labeled following the method described in the BoehringerMannheim DIG labeling kit. ..

Article Title: Purification and characterization of a major collagenase from Streptomyces parvulus.
Article Snippet: .. The PCR product was extracted from agarose gel after electrophoresis, labeled using a DIG Labeling Kit (Boehringer Mannheim, Mannheim, Germany) according to the manufacturer’s instructions, and used as the specific probe for Southern and colony hybridizations. ..

Article Title: Expression and localization of cartilage-specific matrix protein chondromodulin-I mRNA in salivary pleomorphic adenomas.
Article Snippet: Pleomorphic adenoma is the most common epithelial tumor in the salivary glands.. This tumor frequently exhibits “mesenchyme”-like components, including myxoid or chondroid areas.. Recently, using immunohistochemical techniques, we reported that cartilage-specific matrix protein, chondromodulin-I (ChM-I), was deposited on the inter-territorial matrix of the chondroid area in salivary pleomorphic adenomas and that ChM-I, which is also a strong angio-inhibitory factor, plays an important role in the avascular nature of the chondroid area and the chondroid formation in this type of tumor.

Article Title: Role for Macrophage Metalloelastase in Glomerular Basement Membrane Damage Associated with Alport Syndrome
Article Snippet: .. One μg of linear DNA was transcribed as recommended in the Boehringer Mannheim DIG Labeling Kit using T7 polymerase. .. The yield of labeled probe was estimated by spotting the probe and labeled control onto nylon membrane and developing as recommended in the nonradioactive in situ hybridization application manual (Roche Applied Science, Mannheim, Germany).

Article Title: Abundant expression of nucleosome assembly protein 1 (NAP1) gene in goldfish scale with lateral line.
Article Snippet: .. The bi-directional cRNA probes used were synthesized for nucleosome assembly protein 1 (NAP1) using either SP6 or T7 RNA polymerase (Takara, Tokyo, Japan) and the DIG labeling kit (Boehringer, Mannheim, Germany). ..

Article Title: Neonatal Estrogen Causes Irreversible Male Infertility via Specific Suppressive Action on Hypothalamic Kiss1 Neurons.
Article Snippet: .. DIG-labeled antisense cRNA probes for rat Kiss1 (position 33 to 348; GenBank accession no. AY196983),Ghrh (position 184 to 541; GenBank accession no. NM_031577), Pomc (position 206 to 671; GenBank accession no. AF510391), Agrp (position 216 to 646; GenBank accession no. NM_033650), and Gnrh1 (position 57 to 360; GenBank accession number NM_ 012767.2) were synthesized by in vitro transcription from the rat hypothalamic cDNA using a DIG labeling kit (Boehringer Mannheim, Mannheim, Germany). ..

Polymerase Chain Reaction:

Article Title: Purification and characterization of a major collagenase from Streptomyces parvulus.
Article Snippet: .. The PCR product was extracted from agarose gel after electrophoresis, labeled using a DIG Labeling Kit (Boehringer Mannheim, Mannheim, Germany) according to the manufacturer’s instructions, and used as the specific probe for Southern and colony hybridizations. ..

Agarose Gel Electrophoresis:

Article Title: Purification and characterization of a major collagenase from Streptomyces parvulus.
Article Snippet: .. The PCR product was extracted from agarose gel after electrophoresis, labeled using a DIG Labeling Kit (Boehringer Mannheim, Mannheim, Germany) according to the manufacturer’s instructions, and used as the specific probe for Southern and colony hybridizations. ..

Electrophoresis:

Article Title: Purification and characterization of a major collagenase from Streptomyces parvulus.
Article Snippet: .. The PCR product was extracted from agarose gel after electrophoresis, labeled using a DIG Labeling Kit (Boehringer Mannheim, Mannheim, Germany) according to the manufacturer’s instructions, and used as the specific probe for Southern and colony hybridizations. ..

Synthesized:

Article Title: Abundant expression of nucleosome assembly protein 1 (NAP1) gene in goldfish scale with lateral line.
Article Snippet: .. The bi-directional cRNA probes used were synthesized for nucleosome assembly protein 1 (NAP1) using either SP6 or T7 RNA polymerase (Takara, Tokyo, Japan) and the DIG labeling kit (Boehringer, Mannheim, Germany). ..

Article Title: Neonatal Estrogen Causes Irreversible Male Infertility via Specific Suppressive Action on Hypothalamic Kiss1 Neurons.
Article Snippet: .. DIG-labeled antisense cRNA probes for rat Kiss1 (position 33 to 348; GenBank accession no. AY196983),Ghrh (position 184 to 541; GenBank accession no. NM_031577), Pomc (position 206 to 671; GenBank accession no. AF510391), Agrp (position 216 to 646; GenBank accession no. NM_033650), and Gnrh1 (position 57 to 360; GenBank accession number NM_ 012767.2) were synthesized by in vitro transcription from the rat hypothalamic cDNA using a DIG labeling kit (Boehringer Mannheim, Mannheim, Germany). ..

In Vitro:

Article Title: Neonatal Estrogen Causes Irreversible Male Infertility via Specific Suppressive Action on Hypothalamic Kiss1 Neurons.
Article Snippet: .. DIG-labeled antisense cRNA probes for rat Kiss1 (position 33 to 348; GenBank accession no. AY196983),Ghrh (position 184 to 541; GenBank accession no. NM_031577), Pomc (position 206 to 671; GenBank accession no. AF510391), Agrp (position 216 to 646; GenBank accession no. NM_033650), and Gnrh1 (position 57 to 360; GenBank accession number NM_ 012767.2) were synthesized by in vitro transcription from the rat hypothalamic cDNA using a DIG labeling kit (Boehringer Mannheim, Mannheim, Germany). ..



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